Journal: International Journal of Ophthalmology
Article Title: Vascular endothelial growth factor-165b protects the blood-retinal barrier from damage after acute high intraocular pressure in rats
doi: 10.18240/ijo.2022.08.02
Figure Lengend Snippet: The antibodies used in this study
Article Snippet: Retinal sections were observed by confocal microscopy (Olympus FV1000, Japan). table ft1 table-wrap mode="anchored" t5 caption a7 Product Host Company Catalogue number Dilution Anti-VEGF 165b antibody[MRVL56/1] Mouse Abcam ab14994 2 µg/mL for WB; 5 µg/mL for IF Anti-CD31 antibody[EPR17260-263] Rabbit Abcam ab222783 1:100 IF Anti-CD31 antibody[P2B1] Mouse Abcam Ab24590 2 µg/mL for IF Anti-NeuN antibody Rabbit Abcam ab104225 1:500 IF Anti-ZO-1 antibody Rabbit Abcam ab96587 1:1000 WB; 1:500 IF Alexa Fluor 488 anti-rabbit IgG(H+L) Donkey Jackson 711-545-152 1:200 IF Alexa Fluor ® 594 anti-mouseIgG(H+L) Donkey Jackson 715-585-150 1:200 IF Alexa Fluor ® 488 anti-mouse IgG(H+L) Donkey Jackson 715-545-150 1:200 IF Alexa Fluor ® 594 anti-rabbit IgG(H+L) Donkey Jackson 711-585-152 1:200 IF Anti-β-actin loading control Rabbit Abcam Ab1801 1:2000 WB HRP-conjugated anti-mouse IgG(H+L) Goat Abcam {"type":"entrez-nucleotide","attrs":{"text":"Ab205719","term_id":"73912114","term_text":"AB205719"}} Ab205719 1:10000 WB HRP-conjugated anti-rabbit IgG(H+L) Goat Abcam {"type":"entrez-nucleotide","attrs":{"text":"Ab205718","term_id":"73912113","term_text":"AB205718"}} Ab205718 1:10000 WB Open in a separate window WB: Western blotting; IF: Immunofluorescence. caption a8 The antibodies used in this study
Techniques: